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In vitro studies have demonstrated that IL-10 opposes the cellular functions induced by TNF-α and IFN-γ.50 IL-10 knockout mice, which are widely used as a model of spontaneous colitis, show increased intestinal permeability with elevated TNF-α, IL-1β, and IL-6 expression prior to exhibiting histologic signs of intestinal inflammation.51 These observations suggest that IL-10 has a role in the protection of the intestinal barrier